The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
Blog Article
At that time, the early signals have been dismissed as debris, nevertheless the retrospective evaluation demonstrated shared traits Using the problematic cells in culture. Tracing the an infection served the lab build additional laboratory procedures for screening and security of assay-important cell strains.
As samples are obtained within the Attune CytPix Flow Cytometer, the substantial-speed brightfield digicam captures and stores images of detected occasions in a price nearly 6,000 photos per second, based on the flow rate and graphic sizing. For higher versatility, Attune Cytometric Application lets you adjust the picture seize frequency as necessary and choose a particular gate for graphic seize, if demanded.
(B) RAW 264.7 macrophages in many cases are annotated inaccurately with regard to particle rely because of their complexity and noncircular borders. The volume of singlets obtainable for Examination boosts as soon as the person trains and employs a more accurate model.
Find new insights into your experimental information by visualizing populations utilizing merged fluorescence and morphology knowledge. Our software program interface wizards allow downsampling, concatenation (merged documents) and dimensionality equipment which have been built in to the application for an intuitive, streamlined data analysis working experience.
"A picture is just not fairly well worth one thousand dots, nevertheless it seriously helps you to see visuals. It is excellent to view illustrations or photos that correlate with staining. For instance, AnnexinV-good cells which might be morphologically various than nutritious cells, or CD14+ monocytes which are greater and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is actually illuminating to see the number of doublets slip via regular singlet gates, and great to confirm the position of surprising double-beneficial occasions in my Examination."
Pair your Attune flow cytometer using an autosampler for enhanced workflow efficiency, and increase robotic integration for maximum throughput.
Our scientists will layout panels in your case as again and again you will need—totally free, no product order desired.
The Attune NxT cytometer will be the responsible workhorse instrument your lab can rely on for reliable and trustworthy functionality. The Attune CytPix product incorporates a higher-speed brightfield digital camera and AI-driven impression analysis software package, with current updates which includes equipment Finding out applications and an intuitive consumer interface, enabling enhanced information precision, accuracy, and a lot quicker insights into sample biology.
Possibly most significantly, these events have cells of Plainly different phenotypes which may lead to incorrect conclusions concerning double positive situations (especially in scarce populations).
AI-pushed automated graphic Evaluation While using the Attune Cytometric Program interprets celebration characteristics into distinctive morphology parameters which might be combined with regular fluorescence and scatter parameters to reveal really reproducible inhabitants adjustments and exclusive subsets.
Software Take note: Automated impression Examination reduces person-to-user variability in flow cytometry gating tactics
Leverage the strength of State-of-the-art know-how and innovation to accelerate your discoveries. Trust within the Attune Flow Cytometer family members to deliver the trustworthiness, speed, and precision that set your do the job apart.
Flexible payment prepare from Agilent Money Options permit you to obtain the significant instrumentation you'll need for rapid job, or to improve your lab.
At the guts of each Attune Flow Cytometer lies our revolutionary acoustic focusing technology, which utilizes sound waves to meticulously align cells within the flow cell. This impressive technique surpasses common hydrodynamic solutions, causing faster processing speeds, Extraordinary information high-quality, and greater sample throughput.
Because the cell line had previously been analyzed to the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and have been capable to doc the microbial infection not less than five times before.
The Attune CytPix Flow Cytometer makes it possible for customers to visualise samples during acquisition, confirm the accuracy of gating methods, and assist outcomes with reliable documentation for more info publication needs.
In this article, we exhibit that by gating strategies using picture-based quantitative parameters can distinguish interacting cells from coincident events to more properly examine interacting cells.